Abstract: To study the chemical constituents and biological activity of wild agarwood from Aquilaria crassna.Various column chromatographic techniques were used for isolation and purification from the agarwood of A. crassna,and the structures of the compounds were identified on the basis of spectroscopic data and physicochemical properties analysis.Twelve compounds were isolated from the ethanol extract of wild agarwood originating from A.crassna in Cambodia,and the structures were identified as 7α-H-9(10)-ene-11,12-epoxy-8-oxoeremophilane (1),7β-H-9(10)-ene-11,12-epoxy-8-oxoeremophilane (2),neopetasane (eremophila-9,11-dien-8-one) (3),jinkoh-eremol (4),(rel)-4β,5β,7β-eremophil-9-en-12,8β-olide (5),nootkatone (6),11-hydroxy-valenc-1(10)-en-2-one (7),(7S,9S,10S)-(+)-9-hydroxy-selina-4,11-dien-14-al(8),5-hydroxy-2-［2-(4-methoxyphenyl)ethenyl］chromone (9),5-hydroxy-6methoxy-2-(2-phenylethyl)chromone (10),6-methoxy-2-(2-phenylethyl)chromone (11),5-hydroxy-6-methoxy-2-［2-(4-methoxyphenyl)ethyl)chromone (12).Among them,compounds 1-8 are sesquiterpenoids,compound 9 is 2-［2-phenylethenyl］chromone,and compounds 10-12 are 2-(2-phenylethyl)chromone compounds.Compounds 2-11 were isolated from agarwood of A. crassna for the first time.The acetylcholinesterase inhibitory activities of compounds were evaluated by Ellman colorimetric method.Among them,compounds 1~3 and 12 showed acetylcholinesterase inhibitory activities.

Key words: agarwood, Aquilaria crassna, Cambodia;sesquiterpenoids;2 -(2-phenylethyl)chromones, anti-acetylcholinesterase activity