Abstract: To study the chemical constituents and activities of Schisandra sphenanthera Rehd.et Wils..The constituents were isolated and purified with normal phase silica gel chromatography,reversed phase silica gel chromatography and semi-preparative HPLC methods from the bark of dried stems of S. sphenanthera,which were identified by spectral data.They were identified as 6′′-O-p-hydroxybenzoyl salidroside(1),nectandrin B(2),7-(3,4-dimethoxyphenyl)-7′(4′-hydroxy-3′-methoxyphenyl)-8,8′(dimethylbutyl) acetate(3),henricine B(4),(8R,8′R)-3,4-dimethoxy-3′,4′-methylenedioxy-7,7′-dioxo-8,8′-neolignan(5),secoisolariciresinol(6),calceolarioside B(7).Compound 1 is a new compound.Compounds 1,6 and 7 have good inhibitory effects on NO release.

Key words: Schisandra sphenanthera Rehd.et Wils., glycosides, lignans, structural identification, activity study