Abstract: To study the chemical constituents from the fruits of Acanthopanax henryi (Oliv.) Harms based on LPS-induced microglia BV2 as the bioactivity guided model.The compounds were isolated and purified by NP-and RP-silica gel and Sephadex LH-20 column chromatography,as well as Prep-TLC,Prep-HPLC,and recrystallization methods.Their structures were identified on the basis of their physicochemical properties and spectroscopic data.As a result,eighteen compounds were obtained from A. henryi and their chemical structures were identified as 5-hydroxymethyl-2-furaldehyde (1),5-hydroxymaltol (2),protocatechuic acid (3),6-methoxy-7-hydroxycoumarin (4),kaempferol-3-O-β-D-glucoside (5),kaempferol-3-rutinoside (6),(-)-pinoresinol 4-O-β-D-glucopyranoside (7),(+)-simplexoside (8),(-)-sesamin (9),rosin (10),phenylmethyl-β-D-glucopyranoside-6′-O-acetate (11),3,4-dihydroxy-p-menth-1-ene (12),(4R)-p-menth-1-en-4,7-diol (13),(2E,6R)-2,6-dimethyl-2,7-octadiene-1,6-diol (14),(+)-(3S,4S,6R)-3,6-dihydroxy-1-menthene (15),oleanolic acid-3-O-β-D-glucuronopyranoside (16), styraxlignolide E (17),and styraxlignolide D (18),respectively.To the best of our knowledge,compounds 2,8,10-15,17,and 18 were isolated from Araliaceae for the first time.Compounds 1 and 7 were isolated from Acanthopanax Miq. for the first time.Except compounds 4-6,9,and 16,all of the other compounds were obtained from this species for the first time.In addition,the results of screening of anti-inflammatory activity demonstrated that the tested compounds showed a certain NO inhibitory effects,among them,compounds 1,4,7,9,12,13,and 18 showed a moderate NO inhibitory effects.

Key words: Acanthopanax henryi (Oliv.) Harms, flavonoids, monoterpenoids, lignans, chemical constituents, anti-inflammatory activity