Abstract: The spectral-effect relationship between the fingerprints of different polar parts of Saposhnikovia divaricata and the anti-influenza virus activity was investigated,and the effective components of S. divaricata with anti-influenza virus activity were deduced.In this study,the cytopathic effect (CPE) method was used to detect the anti-influenza virus activity of different polar parts of S. divaricata,and the UPLC-Q-Exactive Orbitrap/MS fingerprints of different polar parts of S. divaricata were established;Spearman analysis,gray correlation analysis (GRA) and partial least squares regression (PLSR) analysis were adopted to analyze the spectral-effect relationship between the characteristic fingerprint peaks of UPLC and its anti-influenza virus activity.Among the 28 characteristic fingerprint peaks of calibration,peaks 1,5,7,11,13,20 and 24 were positively correlated with the anti-influenza virus activity,and the corresponding constituents were pyroglutamic acid,divaricatacid,prim-o-glucosylcimifugin,sec-o-glucosylhamaudol,psoralen,isofraxidin and 5-hydroxy-8-methoxypsoralen.The collective results will provide experimental basis for in-depth research on the active substance basis of anti-influenza virus of S. divaricata.

Key words: Saposhnikovia divaricata, UPLC-Q-Exactive Orbitrap/MS, anti-influenza virus, spectrum activity relationship