Abstract: To systemic analysis the chemical profile of Cinobufacini injection using ultra-high performance liquid chromatography electrospray ionization time-of-flight mass spectrometry (UPLC-ESI-QTOF/MS) in MSE mode combined with UNIFI platform.The original MSE data of collected samples and the self-built chemical composition database were imported into UNIFI platform.Then,the chemical constituents of Cinobufacini injection were screened automatically through UNIFI platform and checked one by one through MassLynx workstation.A total of 76 chemical components in Cinobufacini injection were identified,including 36 bufadienolides,12 alkaloids (including a new compound),13 peptides,7 organic acids,4 amides,2 sterols and 2 other components.UPLC-ESI-QTOF/MSE combined with UNIFI informatics platform can quickly separate and analyze the complex natural products,and the results laid a foundation for further study on quality control and toxic-active ingredients of Cinobufacini injection.

Key words: Cinobufacini injection, UPLC-ESI-QTOF/MS, UNIFI, chemical constituents