In order to investigate the effect of tamarixetin on insulin resistance of 3T3-L1 fat cells and the mechanism of AMPK signaling pathway,the 3T3-L1 fat cells were induced by dexamethasone and insulin resistance model was established,then Glu uptake and TG content were detected after administration;Further,qRT-PCR was used to detect the related genes in AMPK signaling pathway,molecular docking was carried out for the related proteins by the docking software,and Western blot was used to protein detection.The results showed that when tamarixetin treated for 48 hours,the Glu uptake had significantly increased in the high and low dose groups (P<0.01),the TG content had significantly decreased in the high dose group (P<0.05);In addition,tamarixetin significantly increased the gene expression of AMPK (P<0.01),decreased the gene expression of FAS (P<0.05),had a good molecular docking with FAS protein,promoted the protein expression of P-AMPK,P-ACC,P-PKB and PPARα,and inhibited the protein expression of FAS.This study provide that tamarixetin can increase the Glu uptake and decrease TG content,which may be related to the regulation of related genes and proteins in AMPK signaling pathway.