Ten compounds were obtained from Fusarium sambucinum B10.2,which was isolated from Aconitum vilmorinianum Kom.Their structures were identified as:cyclonerodiol (1),trichoderiol A (2),T-2 tetraol (3),sambacide (4),12β-acetoxy-4,4-dimethyl-24-methylene-5α-cholesta-8,14-diene-3β,11α-diol (5),bisphenol A (6),β-sitosterol (7),magnolin (8),indazole (9),uracil (10).Compounds 1-3,5-10 were isolated from this fungus for the first time,and 6 was first obtained from nature.The results of bioactivity assays showed that compound 4 could inhibit the growth of Escherichia coli with the minimum inhibitory concentration (MIC) of 32 μg/mL.Compounds 6 and 9 showed strong inhibitory activity against Staphylococcus aureus,and the MIC was 64 μg/mL,which was at the same grade as positive control.Compounds 2,3 and 4 exhibited moderate acetylcholinesterase (AChE) inhibitory effects.