To evaluate the acute toxicity and subacute toxicity of extracts from roots and leaves of Vicatia thibetica de Boiss.in mice.The number and dosage of the drug groups were determined by pre-test,and were administrated by gavage to test the LD₅₀ and MTD of the extracts from roots and leaves of V. thibetica.The subacute toxicity experiments were carried out by gavage of 1/20 and 1/40 of LD₅₀.The results showed that the LD₅₀ of the extracts from the roots and leaves were 154.81 and 58.27 g/kg,and the MTD of the extracts from the roots and leaves were 108.28 and 32.62 g/kg,respectively.There were no significant changes in blood biochemical indexes,organ index,body weight,and food intake in the subacute toxicity experiment group compared with the control group.Therefore,the extracts from the roots and leaves of V. thibetica were of non-toxic grade with high edible safety,and no toxic reaction was found after long-term use.