Bletilla striata polysaccharides (BSP) have attracted extensive research interest due to their potential application of medicinal value and skin care. Herein,enzymatic assisted technique was applied to extract BSP from B. striata of Ta-pieh Mountains for the first time. Firstly,response surface methodology was applied to improve extraction efficiency. Under the optimal conditions,the maximum extraction yield was 64.8%. Scanning electron microscope and spatial conformation analysis shown that enzymatic extraction did not destroy the natural structure of polysaccharides. Then,antioxidant activity of extracted BSP was analyzed,and the results clearance rates of hydroxyl radical,superoxide anion and DPPH were 87.6%,71.5% and 83.9%,respectively,at the finite concentration of BSP,which presented a good antioxidant activity. The extraction yield of BSP was highest report to data. The efficient extraction of the BSP in this study lays the foundation for its industrial application.