To assess the protective effect and underling mechanism of mosloflavone on brain damage induced by high altitude,a brain damage model induced by hypobaric hypoxia (HH) was established and used to evaluate the effect of mosloflavone on oxidative stress and inflammatory response related indexes and proteins in brain issue of mice after high altitude exposure. The results indicated that prior administration of mosloflavone reduced the level of ROS and MDA,elevated the GSH content and activities of SOD,CAT and GSH-Px in brain tissue of mice following HH exposure. In addition,Mosloflavone could decrease the expression of IL-1β and TNF-α and increase the expression of SOD1 and CAT in brain tissue. In summary,mosloflavone exerted its neuroprotective effect on brain injury induced by HH in mice likely through scavenging free radical and inhibiting the release of proinflammatory cytokines,which suppressing oxidative stress and inflammatory response in brain tissue.