The aim of this study is to establish a new extraction method from Pheretima aspergillum,and to investigate its antioxidant and anti-inflammatory activities.The crude was extracted from fresh Pheretima aspergillum by absolute ethanol,purified by MCI column and Sephadex gel LH-20,and Soxhlet extraction was used to decolorization and deodorization.The results showed that the ethanol extract of Pheretima aspergillum obtained in our study was 13.6 g,and the yield was 63.3%,which was the highest yield obtained.TLC results showed that the ethanol extract of Pheretima aspergillum was rich in small molecular compounds with fluorescent properties,and had DPPH free radical,hydroxyl free radical and superoxide anion scavenging activity.It also had higher inhibitory activity on A375 human melanoma cells,and had no significant cytotoxicity on macrophages,but had stronger inhibitory activity on nitric oxide (NO) production.The new extracting method has better separation effect,more convenient operation and higher yield,and the ethanol extract has significant antioxidant,anti-melanoma cells and anti-inflammatory activities.