Mikania micrantha H.B.K is the most invasive plants in the world.The damage has severely affected the economic development of the invaded area;Nine compounds were isolated from the leaves of M. micrantha plants method through silica gel column chromatography,which were methyl tridecanoate (1),(Z)-6-nonadecenoic acid (2),phytyl palmitate (3),palmitic acid (4),α-tocopherolquinone (5),loliolide (6),stigmasterol (7),mikanolide (8),and dihydromikanolide (9) were isolated from leaves of M. micrantha by chromatography method.Their structures were elucidated on the basis of spectroscopic evidence.Compounds 1-6 were isolated from M. micrantha for the first time.The doubling dilution method of broth was used to test the antibacterial activity of compounds 1-9.The results showed that compounds 8 and 9 had antibacterial activity against the tested bacteria,and the minimum inhibitory concentration was 500 mg/L.