In order to better study the bioactive compounds in the chemical research of Vernonia cumingiana Benth, Silica gel and Sephadex LH-20 colunm chromatography were used to isolate the chemical constituents,and seven compounds were isolated and identified through spectroscopic methods (¹H NMR,¹³C NMR,HMBC,HMQC,etc.) chemical methods and X-ray diffraction methods,their structures were established as vernone A (1),α-spinasterol (2),β-sitosterol (3),apigenin (4),tamarixetin (5),fernenol (6),4-hydroxy-2-methoxycinnamaldehyde (7).Compounds 4-7 were isolated from the V. cumingiana for the first time.Compound 1 is a new sesquiterpene compound.The antibacterial activity of the isolated monomeric compound vernone A was determined by double-layer AGAR diffusion method.The results showed that Vernone A had no obvious antibacterial effect on Escherichia coli,Staphylococcus aureus,Bacillus subtilis and Salmonella typhimurium in the experimental concentration range.