To find new compounds in Aconitum forrestii Stapf and enrich the diversity of chemical composition.A new diterpenoid alkaloid along with thirteen known diterpenoid alkaloids were isolated from the ethanol extract of the roots of A. forrestii by silica gel column chromatography.Their structures were identified as 8-O-methyl-14-O-anisoylchasmanine (1),14-acetoxy-8-O-methylsachaconitine (2),14-acetyltalatizamine (3),talatisamine (4),chasmanine (5),ezochasmanine (6),crassicautine (7)，geniculatine C (8)，cammaconine (9)，vilmoraconitine (10)，aconitramine A (11),vilmorrianine G (12),hemsleyaconitine G (13) and heterophylloidine (14) by means of spectroscopic methods such as HR-ESI-MS,1D and 2D NMR.The protective activity against adriamycin induced H9c2 myocardial cell injury of all compounds have been investigated,and the results showed that compounds 3,10,11 and 12 had a certain protective effect.

"> Aconitum forrestii Stapf, roots, isolation and identification, diterpenoid alkaloid, adriamycin, protective activity against H9c2 cardiomyocytes