To study the chemical constituents from the Chloranthus henryi and their anti-inflammatory activities in vitro，various column chromatography techniques such as column chromatography on silica gel,Sephadex LH-20 were used to isolate and purify compounds and their structures were identified by modern spectroscopy techniques including NMR and HR-MS spectroscopic data.Some compounds were evaluated for their inhibitory effects on lipopolysaccharide -induced nitric oxide production in RAW264.7 cells.Nineteen compounds were obtained from the dichloromethane extract from Chloranthus henryi and their chemical structures identified as atractylenolid III（1）,(1E,4Z)-8-hydroxy-6-oxogermacra-1(10),4,7(11)-trieno-12,8-lactone（2）,curcuzederone（3）,curcolonol（4）,(1R,3S,5S,8S,10R)-14-acetylshizukanolide（5）,ent-8(9)-pimarene-20-hydroxy-16-nor-15-oic acid（6）,labda-8(17),13-dien-15,12R-olid-19-oic acid（7）,(11E)-(4S,5R,9S,10S)-15,16-bisnor-13-oxo-8(17),11-labdadien-19-ol（8）,14(15)-bisnor-13-oxolabd-8(17),11(E)-dien-19-oic acid（9）,guaianediol（10）,N-trans-cinnamoyltyramine（11）,N-benzoyl-L-phenylalaninyl-N-benzoyl-L-phenylalaninate（12）,N(N′-benzoyl-S-phenylala ninyl)-S-phenylalaninol benzoate（13）,N-2-phenylethylcinnamamide（14）,N-trans-cumaroiltiramina（15）,5,7,4′-trimethoxyflavanone（16）,2-propenoic acid-3-phenyl-methyl ester（17）,trans-cinnamic acid（18）,trans-p-hydroxycinnamic acid ethyl ester（19）.Compounds 5-19 were isolated form the species for the first time,among which compounds 7 -10,12 and 13 were obtained form genus Chloranthus Swartz for the first time.The in vitro nitric oxide (NO) production inhibitory tests of some compounds showed that compound 2 shows good anti-inflammatory activity with the NO inhibition rate of is 58.74%±8.17% at 10 μmol/L.