In order to explore the effects of leukopenia E,an ent-kaurane diterpenoid on cytoskeleton and cell migration,leukamenin E-induced cytoskeletal rearrangement and inhibition of cell migration were investigated by using MTT,Giemsa staining,flow cytometry and immunofluorescence assay.The results showed that 0.4-1.0 μmol/L leukamenin E inhibited the proliferation of HeLa cells through G₁ arrest,led to obvious change in cellular morphology and nuclear morphology,while reduced the number of pseudopods and increased the number of flattened nucleus.Moreover,the migration of HeLa cells was inhibited.Leukamenin E could significantly alter the arrangement of microtubules and keratin filaments,decrease stress filaments in the cytoplasm,and the aggregation of microtubules and keratin filaments into large bundles around the nucleus have been showed.Leukamenin E induced a significant increase in intracellular ROS levels.A radical scavenger NAC,reduced leukamenin E-induced cell morphology changes,cytoskeletal rearrangement and inhibition of cell migration,namely,these changes was associated with the increase of ROS.These results suggested that upregulation of ROS level induced by leukameninE resulted in the rearrangement of HeLa cytoskeleton by regulating the related signal pathway,which leads to morphological changes of cells and nuclear,and inhibits cell migration.