To explore the mechanism of Shenling Baizhu Powder(SLBZP) in the treatment of colorectal cancer.Using the method of network pharmacology,the active ingredients of SLBZP were collected and screened through the TCMSP database and the targets were obtained.The GEO database was screened to obtain differentially expressed genes between CRC patients and healthy individuals as the relevant targets of CRC.Cytoscape software was used to construct a network of drug components and disease targets.Bisogenet was used to construct a protein interaction (PPI) network to identify candidate targets for SLBZP acting on CRC.Through gene ontology (GO) function enrichment,Kyoto Encyclopedia of Genes and Genomics (KEGG) pathway enrichment analysis was used for biological functions and pathway enrichment of core genes.Cytoscape software was used to constructs a target-pathway network and screen key target genes according to degree.Through analysis,165 core targets of SLBZP for the treatment of CRC were found,and it was found that the functional annotation of core targets was related to transcription factor activity,protein stability regulation,and ubiquitin protein ligase binding.Twenty pathways including PI3K-Akt signaling pathway,PD-L1/PD-1 pathway,and viral carcinogenesis are all significantly enriched.AKT1,TP53,PIK3R1 are the core genes,and MAPK3,NFKB1,CCND1,MAPK1,RELA, CDKN1A,MYC,STAT3,MDM2,JUN,RB1,etc.are the key genes for the treatment of CRC in the SLBZP network pathway.In summary,the therapeutic effect of SLBZP on CRC may be related to specific biological processes and related pathways to regulate inflammatory responses and optimize the structure of intestinal flora to play a therapeutic role.Through network pharmacological analysis and evaluation,the complex mechanism and targets of SLBZP have been further revealed,which is of great significance to the treatment of CRC.