To explore the clinical effect and mechanism of Yangyin Jiedu Decoction on coronavirus disease 2019(COVID-19).The patients with COVID-19 who were treated in general hospital of Guizhou province from January 28,2020,to February 28,2020,were randomly selected as the study object.Their general situation,symptom and signs,liver and kidney function index,nucleic acid Yin conversion and CT imaging changes,before and after the treatment of Yangyin Jiedu Decoction combined routine treatment,were observed.Meanwhile,the network pharmacology and molecular docking was used to analyze the potential targets,components and mechanism of action.Yangyin Jiedu Decoction can improve the clinical symptoms of patients with COVID-19,the network pharmacology analysis showed that 14 potential targets were obtained,56 items were enriched by GO function,and 3 pathways were enriched by KEGG analysis.The results of molecular docking showed that kaempferol,β-sitosterol and marmesin had low conformation energy,stable structure and high binding activity when bind to the receptor protein SARS-CoV-2 3CL hydrolase.Yangyin Jiedu Decoction has a therapeutic effect on COVID-19,which may be related to the antiviral,anti-inflammatory and anti-oxidation effects of kaempferol,β-sitosterol,marmesin and other compounds from various targets.