This article investigated the effect and mechanism of RCE-4 on proliferation,invasion and migration of cervical cancer Ca Ski cells.The effect of RCE-4 on the proliferation of Ca Ski cells was detected by MTT method and clone formation method.The scratch experiment and Transwell chamber experiment were performed to assess the effect of RCE-4 on the migration and invasion of Ca Ski cells.Indirect immunofluorescence experiment and Western blot method were used for detection.The effect of RCE-4 on the transport of β-catenin and YAP/TAZ proteins into the nucleus.Western blot was used to detect the effect of RCE-4 on the expression of Wnt/β-catenin,Hippo/YAP signaling pathway-related proteins and downstream target proteins.The results showed that RCE-4 can significantly inhibit the proliferation,migration and invasion of Ca Ski cells;after RCE-4 treatment,β-catenin and YAP/TAZ proteins enter the nucleus attenuated,and the protein expression of GSK3β,p-β-catenin,p-YAP,p-LATS1 increased,and the protein expression of PAK4, β-catenin,MST1,LATS1,NUAK2,YAP/TAZ,and YAP were decreased,and the expression of the downstream target proteins MMPs,c-Myc,SOX2,CYR61,VCAM-1,ICAM-1 were inhibited,suggesting that RCE-4 may exert its anti-Ca Ski cell invasion and migration effects through regulation of Wnt/β-catenin and Hippo/YAP signaling pathways in vitro.