This study was aimed to establish a UPLC characteristic chromatogram and multi-indicator content determination method for standard decoction of Citrus in Rutaceae,which can provide the basis for its quality evaluation.The UPLC analysis was performed on an Agilent SB C₁₈ column (100 mm×2.1 mm,1.8μm) with the mobile phase consisted of acetonitrile(A) and 0.05% phosphoric acid solution(B) in gradient elution.The flow rate was 0.4 mL/min.The column temperature was 30 ℃.The detection wavelength was 320 nm.Software like similarity analysis,SPSS and SIMCA-P 14.0 were undertaken to analyze 75 batches of Rutaceae standard decoction.The results showed that there were 8 common peaks in Citri Reticulatae Pericarpium Viride standard decoction,7 common peaks in Citri Reticulatae Pericarpium,10 common peaks in Aurantii Fructus,6 common peaks in Citri Grandis Exocarpium and 5 common peaks in Citri Sarcodactylis Fructus.Cluster analysis divided 75 samples into four groups.The standard decoction of Citrus reticulata Blanco,Aurantii Fructus,Citri Grandis Exocarpium and Citri Sarcodactylis Fructus could be distinguished by principal component analysis.OPLS-DA analysis identified different stages of Citrus reticulata Blanco and screened out 4 differential components.Meanwhile,the contents of 7 components were measured.The method established in this study is simple,rapid and reliable,which can provide a reference for the quality evaluation and relevant preparations of Citrus of Rutaceae standard decoction.