Six secondary metabolites were obtained from the rice fermentation extract of endophytic fungus Phomopsis prunorum isolated from Hypericum ascyron L. by various chromatographic techniques.Their structures were elucidated by the analysis of NMR and MS data,including α-pyrone compounds phomopyrones B and C (1 and 2),dibenzo-α-pyrone compounds alternariol (3) and alternariol 9-methyl ether (4),isocoumarin compound (+)-orthosporin (5),and alkaloid compound 3-(2-(4-hydroxyphenyl)-2-oxoethyl)-5,6-dihydropyridin-2(1H)-one (6).Compounds 1 and 2 were new compounds,and compounds 4-6 were isolated from the genus Phomopsis for the first time.The biological activity results showed that compound 3 exhibited moderate α-glucosidase inhibitory activity with an IC₅₀ value of 43.4 μM.However,compounds 1,2 and 4-6 showed no α-glucosidase inhibitory and antibacterial activities.