To study the chemical constituents from the fruiting bodies of Ganoderma lingzhi,all compounds were isolated and purified by various column chromatographies,including silica gel,ODS,Sephadex LH-20 and prep-HPLC,and their structures were identified by NMR,MS and other spectroscopic techniques.Five lanostane-type nortriterpenoids were isolated from 95% ethanol extract of G. lingzhi,which were identified as ethyl 20(21)-dehydrolucidenate A (1),lingzhi-20(21)-en-24-oic acid A (2),20(21)-dehydrolucidenic acid A (3),lucidone A (4),and lucidadone H (5).Compounds 1 and 2 have not been described before,and compounds 3-5 are isolated from this fungus for the first time.Cytotoxic effects of compounds 1-5 were investigated against five regular cancer cell lines,and the results showed that none of them showed significant activity.