EGT (ergothioneine) has a variety of important physiological functions in human body because of its strong antioxidation and low consumption rate.However,the existing EGT detection methods are time-consuming and the reagents & equipments required are expensive in the screening of high-producing EGT microorganisms and the breeding of new strains.In this study,based on the physical and chemical properties of EGT,a high-throughput rapid detection system of EGT-ferric thiocyanate was established.Meanwhile,Ganoderma spp.strains with different EGT production and new Ganoderma spp. fusion strains were selected to verify the accuracy of the detection system.The results showed that this method can quickly and easily determine the difference of EGT yield between samples and shorten the detection time from original 3-4 days to present 2-3 hours.HPLC verification results show that the EGT- ferric thiocyanate high-throughput rapid detection system is effective and stable.The results of this study provided a new method for high-throughput screening of EGT-producing microorganisms and the breeding of new strains with high EGT production.