Isosteroidal alkaloids have variable structures and diverse pharmacological activities,and are mainly distributed in the genera Fritillaria and Veratrum in Liliaceae.In the past 15 years,relevant literatures have reported 80 novel isosteroidal alkaloids.Their pharmacological activities include anti-inflammatory,analgesic,antihypertensive,antiasthmatic, antithrombotic, cholinesterase inhibition,antiviral and antitumor effects.This paper summarized botanical sources,chemical structures and bioactivities of isosteroidal alkaloids from 2006 to 2020.The future research and exploration of isosteroidal alkaloids were discussed and prospected.