The crude polysaccharide was extracted from Nitraria tangutorum Bobr.and precipitated by ethanol then refined by purification through static adsorption and separation.Three main components NTP1,NTP2,NTP3 were achieved by DEAE-Sepharose FF gel filtration chromatography.The composition of monosaccharides determined by PMP pre-column derivation method.In addition,the effects on cell viability,neutral red phagocytoses and NO secretion of RAW 264.7 was carried out.And then,the protein activation/expression of inflammation and oxidative stress signaling pathway were explored.The monosaccharides composition of three NTPs′ monosaccharides consisting mainly of glucose,followed by galactose and glucuronic acid,in addition of few other monosaccharides.NTPs can increase phagocytosis and NO secretion,while NTP1 could activate oxidative stress pathway.NTP2 and NTP3 could activate inflammation pathway.Therefore,the polysaccharides from Nitraria tangutorum Bobr.elicited an activity effect of RAW 264.7 macrophage.