Using green mulberry as raw material,after removing impurities and enriching to obtain crude procyanidin extract,the crude extract was separated and purified by high-speed countercurrent chromatography (HSCCC) and oxidative activity studies.It was concluded that the crude extract of green mulberry procyanidins was separated into five components by HSCCC.Among them,F1 contained catechin,procyanidin B2 and procyanidin A1;F2 contained epicatechin gallate;F3 contained procyanidin B2;F4 contained Epigallocatechin;epicatechin in F5.The 5 components obtained by separation all have certain antioxidant activity,among which the scavenging capacity of F5 and F4 on DPPH radical and ABTS+ radical is close to Vc in the same concentration,and the scavenging capacity on hydroxyl radical is better than Vc in  the same concentration.This paper used HSCCC for the first time to separate and purify proanthocyanidins in green mulberry,which can provide useful technical support for the research and development of active substances in green mulberry.