In order to study the influence of host plants regarding the synthesis and accumulation of metabolites in Taxilli Herba,Ultra-fast liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry (UFLC-Triple TOF-MS/MS) combined with multivariate statistical analysis was used to analyze the differences of chemical constituents in Taxilli Herba from different host plants.Through the analysis of the multistage tandem mass spectrometry,the characteristic peaks were extracted with mass spectrometry data peak matching,peak alignment,and noise filtering.Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) were used to analyze the data.The constituents were identified according to MS accurate mass and MS/MS spectrometry fragmentation information,combined with the software of database search,comparison with reference standards and literature.The results showed that the chemicals constituents in Taxilli Herba from 10 host plants were clearly distinguished.A total of 19 chemical constituents were screened and identified.Among them,4 common differential chemical constituents ((+)-Catechin,quercetin 3-O-β-D-glucuronide,hyperoside,quercitrin) presented different changing laws.Our research may provide basic data for revealing the effect of host plants on the synthesis and accumulation of metabolites of Taxilli Herba and evaluating the quality of Taxilli Herba from different host plants.