Study on the chemical constituents from Tinosporae sinenisis (Lour.) Merr. The shattered stem of T. sinenisis was extracted with 70% ethanol under reflux. The crude extract was extracted with chloroform,ethyl acetate and n-butanol,successively. Thirteen compounds were isolated and purified by silica gel,ODS,Sephadex LH-20 and macroporous resin from chloroform and n-butanol fractions of T. sinenisis. Their structures were elucidated via the interpretation of nuclear magnetic spectrometry,mass spectrometry and other spectral analyses. Thirteen compounds were identified as 2-(2′-hydroxytetracosanoylamino) octadecane-1,3,4-triol (1),1,2-di-O-palmitoyl-3-O-β-D-galactopyranosyl glycerol (2),soyacerebrosides Ⅰ and Ⅱ (3),tinocordiside (4), trans-syringin (5),3,4-dimethoxyphenyl-β-D-glucoside (6),tinosinen (7),5-hydroxymethyl-2-furanacrylic acid (8),tinosineside A (9),tinosposide A (10),isolariciresinol-9-O-β-D-glucopyranoside (11),secoisolariciresinol-9-O-β-D-glucopyranoside (12) and L-pyroglutamic acid (13). Compounds 1-4 were isoalted from chloroform fraction,and compounds 5-13 were isolated from n-butanol fraction. Compound 8 is the first report from natural sources,compounds 1,2,3,6,11 are newly isolated from the family Menispermaceae,compound 13 is newly isolated from T. sinenisis. In addition,all the 13 compounds were evaluated for their inhibitory effects in lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells.