The main mechanism of antioxidation of 10 chemical components in Codonopsis Radix was predicted by network pharmacology method;By constructing an in vitro cell oxidative damage model,To verify the protective effect of the representative active ingredients of Codonopsis Radix screened from network pharmacology on the model.All target proteins of 10 components were obtained through PubMed database and Swiss target prediction website;The related target proteins of oxidative stress were searched by "CTD" database;Using STRING to build a common target interaction network (PPI);Go enrichment analysis of intersection target proteins was carried out through Matescape database.RAW 264.7 cells were injured by H₂O₂.The cells were divided into blank group,H₂O₂ group,Lobetyolin group,L-tryptophan group and syringin group.Using CCK8 method,Kit method,real-time fluorescent quantitative PCR method and Western blot method were used to examine the relevant indicators.214 targets were predicted from 10 monomers targets;881 targets related to oxidative stress were retrieved;20 items involving biological processes,14 molecular functions and 7 cell components were found by Go enrichment analysis.Compared Compared with H₂O₂ group,the cell viability of drug group was enhanced (P<0.05),the activity of antioxidant enzymes and the mRNA expression level of related genes were significantly different,and the expression of related proteins were also significantly different.Network pharmacology research and cell experiment show that the antioxidant stress of active components of Codonopsis Radix can play a role through antioxidant enzymes and Keap1-Nrf2 pathway.