NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2023, Vol. 35 ›› Issue (1): 47-53. doi: 10.16333/j.1001-6880.2023.1.006

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Chemical constituents from the flowers of Bauhinia variegata var. candida and its pancreatic lipase inhibitory activity

PENG Jing1,HE Rui-jie1,XIE Tao-jie2,YANG Bing-yuan1,WANG Ya-feng1,HUANG Yong-lin1*   

  1. 1Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization,Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences,Guilin 541006,China;2Liuzhou Institute of Gardening,Liuzhou 545005,China
  • Online:2023-01-28 Published:2023-02-20

Abstract:

To study the chemical constituents from the flowers of B.variegata var. candida.The constituents were isolated from the ethanol extract of flowers of B.variegata var. candida and purified by column chromatography Sephadex LH-20,MCI and semi-preparative high performance liquid chromatography.The structures of the compounds were identified by NMR spectroscopic data.A total of 17 compounds were isolated from the flowers of B.variegata var. candida,including one glucoside,two benzoic acids,two coumarins,one polycyclic phenols,eleven flavonoids,and the structures were identified as methyl β-D-glucopyranoside (1),methyl-4-hydroxybenzoate (2),4-methoxybenzoic acid (3),p-coumaric acid (4),p-methoxy cinnamic acid (5),pacharin (6),naringenin (7),isovitexin (8),kaempferol (9),6-hydroxykaempfero-3-O-glucoside (10),kaempferol-3-O-α-L-rhamnoside (11),kaempferol 3-O-neohesperidoside (12),kaempferol 3-O-α-L-rhamnopyranosyl-(1-2)-β-D-lucopyranoside (13),quercetin (14),3-O-methylquercetin (15),quercetin-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside (16),quercetin-3-O-(6″-O-α-L-rhamnopyranosyl)-β-D-glucoside (17).All the compounds were obtained from the plant for the first time .Compounds 10,12,13,14,17 were screened for pancreatic lipase inhibitory activity, and the results showed that the five compounds have certain inhibitory activity on pancrelipase.

Key words: Bauhinia variegata var. candida, chemical constituents, structure identification, pancreatic lipase inhibitor

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