In order to study the pharmacodynamic material basis of the Murraya tetramera Huang;in this paper,fifteen compounds were isolated and purified from the ethyl acetate extract of its alcohol extract by modern chromatographic separation technology.And Identified the structure of the compounds by NMR spectroscopy,which were luteolin-7-O-glucoside(1),3,4-dihydroxy benzeneacrylic acid(2),2-hydroxylcinnamie acid(3),3,8-dihydroxy-4-methoxy-coumarin(4),2-methyl-6-hydroxychromone(5),chlorogenic acid(6),methyl caffeate(7),furo［3,2-α］carbazole(8),(E)-2-((4-hydroxyphenyl)diazenyl)phenol(9),N-(2,6-dimethylphenyl)-N′-(4′-nitrophenyl)urea(10),murrayanine(11),phorbazole C(12),3-(2’-hydroxyphenyl)-4(3H)-quinazolidone(13),N-trans-feruloyl-3-methyldopamine(14),6-acetone-5,6-dihydrosanguinarine(15). All compounds are isolated from this plant for the first time.The tumor inhibitory and proliferation effects of 15 compounds were analyzed by the MTS method.The inhibitive effects of the compounds on the growth of five tumor cells in vitro were studied.Especially，the compound 1,8-15 have good growth inhibitory effect on human tumor cell lines HL-60,A-549,SMMC-7721,SW-480 and MCF-7.