Studying the inhibitory effect of different solvent extraction sites of Plumeria rubra (water extract,n-butanol extract,petroleum ether extract,ethyl acetate extract) on degranulation of mast cells.And the mechanism of improving type I hypersensitivity by effective active components of P. rubra was predicated by network pharmacology method and molecules docking.The RBL-2H3 cells were used as the experimental materials,Tween 80 as the model reagent to induce the degranulation of RBL-2H3 cells.The anti-pruritus effects of different extracts were compared.The prediction targets and disease targets of P. rubra were integrated to obtain the key targets.Molecular docking was used to virtual verify the binding ability between the effective active components of P. rubra and core targets.The cell experiment showed that the ethyl acetate extract of P. rubra had obvious effect on the anti-hypersensitivity.And the anthocyanins and flavonoids of treating hypersensitivity in P. rubra may be related to D(2) dopamine receptor,P2Y purinoceptor 2,5-hydroxytryptamine receptor 2A,P2Y purinoceptor 12,muscarinic acetylcholine receptor M1,5-hydroxytryptamine receptor 2C,muscarinic acetylcholine receptor M2,alpha-1D adrenergic receptor,alpha-1A adrenergic receptor,P2Y purinoceptor 1.