To explore the mechanism of Momordica charantia in the treatment of diabetes nephropathy (DN) by network pharmacology and molecular docking.The Herb database was used to obtain the active components of M. charantia,and the SwissTargetPrediction database was used to obtain corresponding targets.Get the therapeutic targets of diabetes nephropathy through GeneCards,OMIM and DisGENT databases.Venn online tool was used to obtain the common targets of the active ingredients of M. charantia and diabetes nephropathy.The interaction network diagram of M. charantia,active ingredients and targets is constructed by using the Cytoscape software,and the core target and core subnet are obtained by using the CytoHubba plug-in.The Matescape database was used for gene ontology (GO) enrichment analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis.Finally,the active ingredients of M. charantia,diabetes nephropathy,and the relationship between them were comprehensively analyzed and predicted.In this study,eleven active components with action targets were screened from M. charantia,among β-glutosterol,momordic acid and lycopene can obviously improve diabetes nephropathy.Simultaneously,the possible targets for regulating diabetes nephropathy,such as AKT1,TNF,IL6,TP53,VEGFA,JUN,CASP3,were predicted to mediate AGE-RAGE signaling pathway,IL-17,TNF,PI3K-Akt,HIF-1 signaling pathways to regulate diseases,so as to combat the occurrence and progress of diabetes nephropathy.The network pharmacology was used to explore the characteristics of multi-component,multi target and multi pathway of M. charantia in the prevention and treatment of diabetes nephropathy,and explore the possible targets and signal transduction mechanisms of the active components of M. charantia in the regulation of diabetes nephropathy.