To investigate the target of julibroside J₈ (J8) inhibiting the proliferation of tumor vascular endothelial cells and its related signal pathway of apoptosis.In this paper,HPLC method was used to detect the content change of J8 in and out of endothelial cells after being treated with J8;Vina software docked J8 with VEGF,FAS,DR3,DR4,DR5,TFR-1 for analysis;Western blot method was used to detect the changes in the expression levels of VEGF,p-JNK,Bax,ENDOG,Caspase-3,Caspase-8,and Caspase-9 proteins in endothelial cells before and after drug addition.The results of molecular docking showed that the target proteins corresponding to VEGF and FAS had good binding ability to J8 and could bind to multiple sites.After HUVEC cells were treated with J8 for 24 h,the expressions of VEGF,p-JNK and other proteins were significantly decreased,and the expressions of apoptosis inducing proteins Bax and EnDOG were significantly up-regulated,and there was no significant effect on the expression levels of Caspase-3,Caspase-8 and Caspase-9.J8 may inhibit the proliferation of vascular endothelial cells by binding with VEGF on the surface of vascular endothelial cell membrane,and cause endothelial cell apoptosis by weakening the activity of VEGF/JNK pathway.