In order to clarify the content of main nutrients in the leaves of Ilex vomitoria to carry out the diversified utilization, this study took young leaves of I. vomitoria in spring, mature leaves in summer and old leaves in autumn as materials, and used freeze-drying method to measure and analyze the content of mineral elements, soluble sugars, soluble proteins, crude fats, vitamin C and amino acids in leaves in different seasons, providing scientific basis for its development and utilization.The results showed that:the leaves of I. vomitoria were rich in mineral nutrients.The content of mineral elements in spring leaves was the highest, and the contents of N, P, Fe and Mn were significantly higher than those in summer and autumn (P < 0.05).The soluble sugar, soluble protein and crude fat in the leaves of I. vomitoria in spring were the highest, and significantly higher than those in summer and autumn.The highest total amino acid content in the leaves of I. vomitoria was 1 645.139 μg/g in spring, followed by 1 425.104 μg/g in summer, and the lowest was 554.806 μg/g in autumn.The highest essential amino acid in leaves was 571.620 μg/g in spring, followed by 394.809 μg/g in summer, and the lowest was 225.185 μg/g in autumn.Analysis of variance showed that there were significant differences in amino acid content of I. vomitoria leaves in different seasons.At the same time, principal component analysis was used to extract two principal components, and a comprehensive evaluation model was established according to the score value of each principal component and variance contribution rate.The comprehensive scores of amino acid content of I. vomitoria leaves in three seasons were obtained, which were summer, spring and autumn in order from high to low.In summary, the spring leaves of I. vomitoria contain relatively rich mineral elements and soluble sugars, making it a suitable period for picking, processing, and utilization.