To establish an UPLC method for the fingerprint analysis and content determination of standard decoction of Rosae Chinensis Flos (RCF),and to provide reference for quality control of standard decoction of RCF,the fingerprints of 20 batches of standard decoction of RCF was established by ultra-high performance liquid chromatography (UPLC),and the common fingerprint peaks were identified by mass spectrometry analysis and comparison with reference materials.The similarity evaluation combined with chemical pattern recognition were applied to explore characteristic components affecting the quality of standard decoction of RCF,and the content of eight components in the main fingerprint peaks were determined simultaneously.Results the UPLC fingerprint of standard decoction of RCF was established,and 15 fingerprint peaks were identified,and the similarity of 20 batches of samples was 0.983-0.999.Hierarchical cluster analysis (HCA) and principal analysis (PCA) divided the samples into four categories,and combined with partial leats square discriminant analysis (OPLS-DA) to screened out 11 signature compounds,of which eight components were confirmed by mass spectrometry and control products.The results showed that the content of gallic acid,ellagic acid,isoquercetin,astragalin and quercetin in the standard decoction of RCF from Zhoukou,Henan Province were significantly higher than those from other areas.The analysis method established of standard decoction of RCF is stable,feasible and reproducible,which can provide a reference for the relative preparation development and quality standard research of standard decoction of RCF.