In order to explore the antioxidant chemical constituents of total flavonoids from stems and leaves of Chrysanthemum morifolium (TFCSL),and to reveal its pharmacodynamic material basis and mechanism of action.In this study,HPLC was used to establish the fingerprints of different batches of TFCSL;high glucose concentration induced oxidative damage model in human umbilical vein endothelial cells,and malondialdehyde,lactate dehydrogenase and superoxide dismutase activities were used as indicators of drug efficacy;gray correlation and partial least squares were used to analyze the spectral-efficacy relationship to determine the antioxidant efficacy substances of TFCSL;based on network pharmacology combined with molecular docking to explore the core targets and pathways of action.Twelve common peaks were identified from the fingerprints of 12 batches of TFCSL,and 9 chemical components were designated;all batches of total flavonoid samples reduced apoptosis,lowered malondialdehyde and lactate dehydrogenase content,and increased superoxide dismutase activity;peak 5 (apigenin-7-O-β-D-glucoside),peak 6 (isochlorogenic acid C) and peak 7 (diosmetin-7-O-β-D-glucoside) were selected as the basis for TFCSL antioxidant substances by integrating two mathematical models;the three screened active ingredients acted on 33 targets of anti-oxidative stress;the key targets were TNF,CASP3,EDNRA,XDH,PTGS2,MMP2,etc.,which were mainly involved in signaling pathways such as lipid and atherosclerosis signaling pathways,IL-17 signaling pathway,diabetic complications AGE-RAGE signaling pathway,TNF pathway,MPKA pathway,and other signaling pathways;the molecular docking results showed that there was good binding between the active ingredients and the key targets.It indicates that the material basis of TFCSL anti-oxidative stress may be apigenin-7-O-β-D-glucoside,isochlorogenic acid C,diosmetin-7-O-β-D-glucoside,and it is speculated that it plays a role in the role of atherosclerosis signaling pathway and IL17 signaling pathway through the targets of TNF and CASP3,reflecting the role of multi-components and multi-targets of stems and leaves of Chrysanthemum morifolium in the function of anti-oxidation.