At present,the research on the isolation and purification of oleuropein mainly were focused on macroporous adsorbent resin,but there were few reports on the isolation and purification of oleuropein with anionic resin.To study the adsorption and separation process of anionic resin (LX-68M),adsorption kinetics and isothermal adsorption models were used to investigate the static adsorption and desorption behavior of oleuropein by anionic resin.The results showed that the adsorption of anionic resin on oleuropein was accordance with the pseudo-second-order kinetic model (R²=0.993 4) and the Freundlich isothermal adsorption model (R²=0.964 2).Further,the separation and purification of oleuropein through the anionic resin was optimized by dynamic experiments,and the optimal operating process was as follows:the extraction solution concentration was 0.25 g/mL,the desorption solution was 45% ethanol,and the adsorption and desorption rates were 2.0 BV/h.The adsorption and desorption rates of anionic resin for oleuropein under these conditions were 86.86% and 87.08%,respectively,and the purity of oleuropein was 52.33%.In addition,the adsorption rate of the anionic resin was 54.9% after four times of repeated use and recovered to 93.51% after regeneration treatment,indicating that it has prospect for industrialization.This anionic resin has good stability and mild separation conditions,and is suitable for the isolation and purification of oleuropein and other similar natural products.