To study the chemical constituents of the aboveground of Chrysanthemum indicum L. All compounds were separated and purified by MCI,ODS,SephadexLH-20 gel column chromatography and pre-HPLC,and the structures of them were identified by analyzing of their spectroscopic data.Compounds 1-6 were isolated from the dichloromethane extract of C. indicum, which were identified as chrygermalide A (1),3β-hydroxy-oxo-7α,11β-germacra-4Z,10(14)-diene-12,6α-olide (2),deacetylherbolide A (3),9β-hydroxy costunolide (4),3β-hydroxycostunolide (5),3β-hydroxy-11α,13-dihydrocos-tunolide (6).Compound 1 is a new compound,and 2-6 are isolated from this genus for the first time.The anti-inflammatory activity of compounds 1-6 were tested in LPS-induced RAW 264.7 macrophage cells,and the IC₅₀ values of their NO release inhibition were 17.9-33.9 μmol/L.