To investigate the chemical composition of the medicinal plant Rosa laevigata Michx derived endogenous fungi (Diaporthe sp. JH-1),we used a combination of multiple approaches,including silicon,ODS and gelatin rod chromatography,semi-prepared high-performance liquid chromatography (HPLC),high-resolution mass spectrometry,and magnetic resonance imaging (NMR).As a result,six chitin derivatives,including diaporisoindoles A,B,and E (1-3),and diaporindenes B-D (4-6) were isolated and characterized.These compounds were isolated from the genus Diaporthe sp.JH-1 for the first time.In vitro anti-inflammatory activity testing of compounds 1-6 revealed significant inhibition of NO production by compounds 1 and 3.Further investigation uncovered that 1 and 3 exert strong inhibitory effect on COX-2,PEG2,and its mechanism was related to the suppression of NF-κB.