The GC-MS analysis of bergamot essential oil was performed to obtain the aroma component content and main aroma substances.The bergamot essential oil concentration was selected by sensory experiments.The EEG experiment and facial expression experiment were carried out in three groups:blank group,unilateral component group and essential oil group,and anxiety relief research was carried out.The results showed that a total of 41 aroma components were analyzed by GC-MS,including limonene and linalyl acetate,and linalyl acetate was selected as a single component.Thirty students majoring in flavor and fragrance from Shanghai Institute of Technology were found for sensory experiments,and the concentration of 3‰ was selected as the concentration of the subsequent essential oil group.In the EEG experiment,the sniffing single-component group and the essential oil group were both alpha wave increased,Beta wave decreased,and the anxiousness relief effect was sorted as essential oil group> single-component group > blank group.In the facial expression experiment,the single-component group had no significant effect on alleviating anxiety,and the essential oil group had obvious micro-expression changes.The overall result was that the essential oil group had the best effect,and the linalyl acetate single-ingredient group had a slight effect.