The anti-inflammatory constituents from ethyl acetate extract of Ipomoea turbinata seeds were studied on the basis of lipopolysaccharide-induced mouse mononuclear macrophage RAW 264.7 cell model.Fifteen compounds were isolated from active EtOAc extract by silica gel,Sephadex LH-20,MCI,ODS column chromatography and semi-preparative high performance liquid chromatography (HPLC),and their structures were confirmed by modern spectral techniques and physicochemical properties,as ipalbidine (1),ipalbidinium-4′-O-β-D-(6-O-trans-coumaroyl)-glucoside (2),1,8,15,22-tetraazacyclooctacosane-2,9,16,23-tetraketone (3),1,8,15,22,29-pentaaza-pentadecane-2,9,16,23,30-pentanone (4),methyl chlorogenate (5),chlorogenic acid (6),3,5-dicaffeoylquinic acid (7),3,4-dicaffeoylquinic acid (8),caffeic acid (9),3,5-dihydroxycinnamic acid (10),4-methoxy-cinnamic acid (11),6-O-(E)-p-hydroxycinnamoyl-1-β-ethyl-glucopyranoside (12),E-3-(3,4-dihydroxy benzylidene)-5-(3,4-dihydroxy phenyl)dihydrofuran-2-one (13),yringaresinol-4-O-β-D-glucopyranoside (14) and pinoresinol 4-O-β-D-glucopyranoside (15).Compounds 3-8 and 10-15 were isolated from this species for the first time,in which eight compounds (3-5,10-13 and 15) were isolated from the Convolvulaceae family for the first time.Bioassay results showed that caffeoylquinic acids (5-8) and phenylpropionic acids (9-12) showed relatively good NO inhibitory activities,with the inhibition rate of 29.07%-40.30% at 50 μmol/L.