Effect of Julibroside J8 on Apoptosis of Human Microvascular Endothlial Cell

Abstract

Abstract:

HMEC-1 cells were treated with different doses of julibroside J8 and different time. The inhibition of cell growth was detected by using SRB assay. The TUNEL and DAPI fluorescent staining were measured in cells.Annexin-Ⅴ/PI double staining was used to measure the cell apoptosis rate by flow cytometry. The results showed that julibroside J8 could inhibit the growth of HMEC-1 and induce the cell apoptosis. The biggest inhibition rate was up to 75.6% and the apoptosis rate was about 32.32%, respectively.

Key words: Julibroside J8, HMEC-1, apoptosis

CLC Number:

HUA Hui, FENG Lei, ZHANG Xiao-Ping, ZHANG Lian-fen, JIN Jian. Effect of Julibroside J8 on Apoptosis of Human Microvascular Endothlial Cell[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(3): 432-435.

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