Abstract:

The polysaccharide of Potentilla anserine L. was extracted and purified, and its contents was determined as 98.4% by phenyl-sulfuric acid method. The polysaccharide was identified by IR spectrum and UV scanning spectrum. The IR spectrum indicates that the characteristic absorption peaks at 3600-3200, 3200-2800, 1400-1200, 1200-1000 cm-1, and 845 cm−1 belonged the characteristic peak of α-pyranose. In addition, the peak at 1630 cm−1 assigned to the C=O of acetamide moiety stretching vibration meant that amino sugars exited in the polysaccharide. A method was developed for the separation of derivatized carbohydrates of P. anserine using 1-naphthyl-3-methyl-5-pyrazolone (NMP) as derivatization reagent by capillary zone electrophoresis, and the results shows that the monosaccharides compositions of the polysaccharide from P. anserine are xylose, arabinose, glucose, rhamnose, mannose, fucose, galactose, glucuronic acid and galacturonic acid with contents of 3.945, 77.445, 17.568, 17.646, 3.942, 2.165, 65.268, 13.037, and 33.484 μg/mg, respectively, which are consistent with the results carried out by GC-MS.

Key words: Potentilla anserine L., polysaccharide, spectral properties, monosaccharide composition