Abstract:

In order to inveatigate the interaction between rutin and human serum albumin (HSA), this article determined the ultraviolt-visible (UV-Vis) absorption spectrum, circular diachroism and HSA fluorescence spectrum, and further to analyse the difference between the spectra before and after rutin and HSA mixed. The results indicated that rutin shows three particular UV absorption-peak at the wavelength 264.0, 285.5 and 354.5 nm and circular diachroism at the wavelength ranges 330~300 nm and 300~230 nm. When rutin interacted with HSA, the UV absorption peak of Rutin will be shifted to long wavelength. However, for the structure of HSA, rutin will change the HSA tertiary structure rather than secondary strcture. The fluoresence of HSA is also influenced by rutin. The excitation wavelength will be shift towards long wavelength, while emission wavelength will be shift towards short wavelength when HSA interacted with rutin.

Key words: Human Serum Albumin (HSA), UV-Vis absorption, Circular Diachroism, fluorescence