Abstract:

A method was established for determining five saikosaponins simultaneously, including saikosaponins a, c, d, e and f in the root of Bupleurum yinchowense by HPLC for the first time. Separation was achieved by A Hibar® RT C18 column (250 mm×4.6 mm, 5 μm) using acetonitrile and water as the mobile phase with gradient elution at the f1ow rate of 1 mL/min, column temperature at 30 ℃ and the wavelength of UV detection was 210 nm.There were good linear correlations between the studied concentrations of the five saikosaponins and their chromatographic peak areas, and the correlation coefficients were 0.9992-0.9998.The recoveries of saikosaponins a and d were 101.5 and 98.5%, respectively. The RSD of precision and accuracy was less than 3%. This method is simple, accurate and reproducible, and also provides a reference for establishing the multiple-value quality control method for Radix Buplerui and the use of B. yinchowense as a medicine.

Key words: HPLC, Bupleurum yinchowense, saikosaponin a, saikosaponin c, saikosaponin d, saikosaponin e, saikosaponin f