Mutation Breeding of High-yield Lipase Strain and Properties of Lipase

Abstract

Abstract:

The mutation strain DX213 was obtained by UV and microwave treatment from Saccharomyces cerevisiae. Its enzymatic activity was 635 U/mL, enhanced by 69%. The genetic characters were stable after enrichment culture for five generations. The optimal conditions for producing lipase were culture temperature of 30 ℃ and pH of 7.5. Enzymatic properties were studied and found that the optimal temperature and pH for the lipase were 40 ℃ and 7.5, respectively. The enzymatic activity kept stable below 40 ℃. Fe³⁺had an activating effect on lipase. The enzymatic activity went up to 720 U/mL when the Fe³⁺concentration was 0.04 g/mL.

Key words: microwave, strain, activity, Saccharomyces cerevisiae, mutation

CLC Number:

DENG Xin, FANG Zhen, ZHANG Fan, LONG Yun-duo, ZENG Hong-yan. Mutation Breeding of High-yield Lipase Strain and Properties of Lipase[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(3): 512-516.

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