NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2011, Vol. 23 ›› Issue (3): 542-546.

• Article • Previous Articles     Next Articles

Extraction of Ceramide from Konjac and Its Sphingomyelin Analysis

LIU Ren-ping1, YANG Jian-kui2, YAN Yan-na1, WU Min-xi1, XIAO Wu-xuan3, WU Yong-yao1*   

  1. 1College of Bioscience and Biotechnology, Hunan Agriculture University; 2Science of College of Hunan Agriculture University; 3Hunan Academy of Agriculture sciences, Changsha 410128, China
  • Received:2009-09-07 Online:2011-06-25 Published:2011-08-08

Abstract:

The extration of ceramide from konjac by the refluxing method was studied. The effects of temperature, time, proportion of solute to solvent and extracting solvent on the extraction efficiencies were analyzed. The optimum extraction conditions were made. Combined with HPLC and evaporative light scattering detector (HPLC/ELSD), The sphingomyelin of konjac were analyzed qualitatively and quantitatively.

Key words: konjac, sphingomyelin, HPLC-ELSD

CLC Number: