Determination of Cimicifugoside H-1 in Rhizoma Cimicifugae by RP-HPLC

Abstract

Abstract: To establish an RP-HPLC method for determination of cimicifugoside H-1 in Rhizoma Cimicifugae. H&E XP ODS-A (4.6×250 mm, 5 µm) column was employed with acetonitrile-water-phosphoric acid (35:65:0.4) as mobile phase, the flow rate was 1.0 mL/min. The column temperature was set 26 ℃ and detection wavelength was set at 203 nm. The linear range of cimicifugoside H-1 was 0.95~28.5 µg, the correlation coefficient was 0.9999 and the average recoveries (n=6) was 99.35%. The method is simple, rapid and reliable, which can be regarded as a reasonable method for determining the content of cimicifugoside H-1 in Rhizoma Cimicifugae. The results can be supported as data bases for developing quality specification of Rhizoma Cimicifugae.

Key words: Rhizoma Cimicifugae, cimicifugoside H-1, HPLC, quantitative determination

CLC Number:

R284.1
R917

YAO Mei-fen, WANG Yue-feng, LI Zhan, PAN Rui-le, ZHAO Xiao-hong, SI Jian-yong. Determination of Cimicifugoside H-1 in Rhizoma Cimicifugae by RP-HPLC[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(4): 696-699.

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Determination of Cimicifugoside H-1 in Rhizoma Cimicifugae by RP-HPLC

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