NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2011, Vol. 23 ›› Issue (1): 72-75.

• Article • Previous Articles     Next Articles

Extraction and Isolation of Pepsin-solubilised Collagen from Jellyfish (Rhopilema esculentum)

LIU Lei, CHEN Wei*   

  1. Laboratory of molecular biology, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, China
  • Received:2009-04-03 Published:2011-07-20

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Abstract:

Pepsin-solubilised collagen (PSC) without was isolated from the mesogloea of jellyfish (Rhopilema esculentum), by limited pepsin digestion and isolated by selective precipitation with NaCl and cm-cellulose chromatography. The PSC exhibited a maximum absorbance at 235 nm. The results of SDS-PAGE and amino acid compositions suggested the purified collagen was type I collagen. It was the same with those of the collagen from jellyfish umbrella and the acid-soluble collagen from its mesogloe, such as S.nomurai and Rhopilema asamushi. As a primitive animal, amino analysis of psc revealed the slight difference between invertebrate and vertebrate.

Key words: jellyfish, collagen, extraction, isolation, amino acid compositions